Program

Oral Sessions

Day 1, May 15（Tue.）　17:20-17:40　Room A (OrBit Hall)

1A-O2-M-1720　　PDF

Inter-laboratory comparison of metabolite measurements for metabolomics data integration

(¹Kyushu Univ., ²Osaka Univ., ³Keio Univ., ⁴RIKEN, ⁵Univ. Tokyo, ⁶Eisai)
Yoshihiro Izumi¹, Fumio Matsuda², Akiyoshi Hirayama³, Kazutaka Ikeda⁴, Yoshihiro Kita⁵, Kanta Horie⁶, Takeshi Bamba¹, ^oYoshiya Oda^5,6

One of the current problems in metabolomics is the difficulty in integrating data collected on different days, using different equipment and/or facilities. Accordingly, the development of a fundamental methodology that enables quantitative comparison of large scale data is needed for next-generation metabolomics study. As a starting point, we examined whether different analytical methods among different laboratories provide within tolerant relative quantification data for certain metabolites. Identical samples extracted from two human cancer cell lines (HeLa and MCF-7) were distributed to each facility, and hydrophilic and hydrophobic metabolites analyses were performed. Based on the preliminary results, we investigated the number of identified metabolites from cancer cells and the variations of relative quantification results (MCF-7/HeLa) among analytical methods.