3D-O2-1625 PDF
Nano-PALDI MSイメージング法による残留農薬の可視化
The use of pesticide under government restrictions is required to increase yields and quality of crops such as fruit, vegetables, wheat, and rice. Procymidone is often used during the summer cultivation of cucumbers to prevent mold growth.
Cucumbers are an important vegetable with in high demand in the world market. Products are scientifically tested for safety to avoid improper application of procymidone and undesirable procymidone residue in commercial cucumber products. Because procymidone is toxic to microorganisms, the pesticide residue is not degraded as a xenobiotic and is retained in plant tissues. Most countries have established maximum procymidone residue limits for agricultural products.
Of particular concern is whether the pesticide diffuses into cucumbers after spraying. To solve this problem, visual information is more useful than chromatographic data and provides an anatomical distribution of the pesticide inside cucumber. To determine target molecules visually in a single measurement, direct analysis of the target small molecule in a sample has been performed by nano-PALDI based mass spectrometry imaging (MSI). In this study, we analyzed the dynamics of the penetration of procymidone residue in cucumbers by chromatography and MSI. This is the first report of dynamic images of pesticides in cucumbers after spraying with an adequate amount of procymidone.