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第3日 5月19日(金) 9:50~10:10 B会場(102)

Mapping of the human cancer tissue glycome of 20 tumor types using MALDI-FTICR and rapid MALDI-TOF mass spectrometry imaging

(1MUSC2Bruker)
oDrake, Richard1Mehta, Anand1Bruner, Evelyn1Cornett, Dale2Angel, Peggi1

We have recently developed a MALDI imaging mass spectrometry method to spatially profile N-linked glycans in frozen and formalin-fixed paraffin-embedded tissue sections and tissue microarrays. Processed FFPE tissues are incubated with a molecular coating of peptide N-glycosidase, and released N-glycans are detected by MALDI imaging mass spectrometry, linked directly with tissue histopathology. Two custom tissue microarrays comprised of multiple tissue core pairs of non-tumor and tumor regions representing twenty different tumor types were processed for N-glycan imaging by MALDI-FTICR and a new MALDI-TOF, rapifleX. The approach allows comparison of the similarities and differences in N-glycome compositions across each tumor tissue type, as well determine the relative abundance of each individual glycan. Substantial increases in intratumoral paucimannose and high mannose glycans was common for many tumor types. Using hierarchical clustering and other methods, differences in branching complexity, bisecting N-acetylglucosamine, and the degree of fucosylation and sialylation were also readily evaluable. Additionally, data from each of the tissue microarrays are being compared to the N-glycans detected in the source tissues. The goal is to generate an extensible reference database of N-glycans for each tumor tissue type, linked with histopathology, as well as develop new cancer biomarker panels.