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第2日 5月18日(木) 10:30~10:50 C会場(101)

Inclusion-list based data dependent acquisition MSによる効率的糖ペプチド解析

(1横市大院生命医2横市大国際総合)
o太田悠葵1吉田晴香1川崎ナナ1,2

We developed an inclusion-list based data dependent acquisition for effective glycopeptide analysis by LC/MS/MS. An aliquot of glycopeptides was digested by endoglycosidase F1, F2, and F3 which cleave chitobiose cores. The resulting peptide+GlcNAcs have tendencies to be eluted near original glycopeptides. Peptide+GlcNAcs were identified by preliminary reversed phase (RP)-LC/MS/MS. We prepared an inclusion list containing retention times and m/z of original glycopeptides estimated by the preliminary analysis. Product ion scans of original glycopeptides were conducted with high efficiency using the inclusion list.