演題概要

ポスター発表

第3日 5月20日(金)  ポスター会場(月光)

標的ペプチド・データベースを用いた内在性ペプチド同定ソフトウエアの開発

(島津)
o村瀬雅樹福山裕子日置雄策山田賢志児嶋浩一岩本慎一田中耕一

Mass spectrometry is promising technology for peptidomic analysis. However peptide identification by conventional DB searching is challenging, since non-specific processing and PTMs of endogenous peptides results in very large search space, yielding poor results. To decrease the search space, we introduced targeted database containing previously identified peptide sequence with high confidence and its PTM sites and developed novel searching software which enables to identify sibling modified peptides of registered peptides in the targeted database. As a result of preliminary test for small set of MALDI MS2 spectra from human urinary peptide mixture, our approach showed high specificity and sensitivity using targeted peptide database which is generated from public human urinary peptide database.