演題概要

オーラルセッション

第3日 5月20日(金) 10:30~10:45 C会場(星雲2)

13C代謝フラックス解析法の精度向上のためのGC-MS/MSによる新規アミノ酸フラグメントイオンの探索

(1阪大情報2島津3阪大工)
o岡橋伸幸1川名修一2飯田順子2,3清水浩1松田史生1

Novel fragmentations of tert-butyldimethylsilyl (TBDMS)-amino acids were investigated by gas chromatography-tandem mass spectrometry (GC-MS/MS). The product ion spectra of 15 TBDMS-amino acids revealed 24 novel fragment ions. The amino acid-derived carbons included in the five fragment ions were identified by the analyses of 13C-labeled authentic standards. It was also confirmed that the precision of 13C-metabolic flux analysis in Escherichia coli central carbon metabolism could be improved by introducing 13C-labeling data derived from novel fragmentations.