演題概要

ポスター発表

第２日　５月１９日（木）　　ポスター会場（いちょう）

2P-41　　PDF

ステロイドホルモンのイオン化法の改良と質量分析イメージングへの応用

(¹奈良医大・ ²京大化研・ ³医薬基盤研・ ⁴茨城医療大・ ⁵関大化学生命工・ ⁶横国大・ ⁷大教大)
^o秦野修¹・ 磯崎勝弘²・ 竹森洋³・ 大西健⁴・ 五百崎太輔⁵・ 森本翔大⁶・ 一柳優子⁶・ 川崎英也⁵・ 片桐昌直⁷・ 荒川隆一⁵・ 車谷典男¹

Mammalian steroid hormones are synthesized mainly in adrenal cortex and gonads (testis and ovary) and function as glucocorticoids, mineralocorticoids, and male- or female-hormones. These steroid hormones are synthesized by several cytochrome P450s and hydroxysteroid dehydrogenases. Since the ionization efficiency of steroid hormons is generally low, we tried to improve the ionization methods of steroid hormones mainly for the application to MALDI mass spectrometry. Several derivatization reagents and metal nanoparticles including commercially available- and newly synthesized-materials were useful for the high efficiency detection of steroid hormones. We also applied theses reagents to Imaging Mass Spectrometry of steroid hormones using mainly FT-ICR MS (Bruker: Solarix).
This work was supported by the Collaborative Research Program of Institute for Chemical Research, Kyoto University (grant #2015-103, 2016-102).