演題概要

ポスター発表

第２日　５月１９日（木）　　ポスター会場（月光）

2P-03　　PDF

グルタチオンへのマイケル付加反応を利用したタバコ煙中のα,β不飽和カルボニル化合物の同定

(¹武庫女大薬・ ²理研・ ³横市大)
^o堀山志朱代¹・ 本田千恵¹・ 市川厚¹・ 畑井麻友子¹・ 吉川紀子¹・ 中村一基¹・ 國友勝¹・ 江崎剛史²・ 升島努²・ 高山光男³

Cigarette smoke contains many toxicants that cause oxidative damage in various cells and tissues. We have previously detected three α,β-unsaturated carbonyl compounds, methyl vinyl ketone (MVK), acrolein and crotonaldehyde, in aqueous nicotine/tar-free cigarette smoke extract (CSE). These compounds as well as CSE irreversibly modified glutathione (GSH), an important antioxidant, by the Michael addition reaction in mouse melanoma cells, causing a rapid depletion of intracellular GSH and leading to cell death.
In this study, we tried to presume the structure of other α,β-unsaturated carbonyl compounds contained in the CSE, by measuring the elemental composition of the Michael addition reaction products of CSE and GSH.