演題概要

ポスター発表

第１日　５月１８日（水）　　ポスター会場（月光）

1P-24　　PDF

翻訳後修飾ペプチドの構造解析を志向した高エネルギー電子移動解離（HE-ETD）が可能な高分解能タンデム飛行時間質量分析装置(TOF/TOF)の開発

(¹阪府大院理・ ²阪大院理)
^o早川滋雄¹・ 曽木政伸¹・ クズミンアレクシー¹・ 藤原亮正¹・ 今岡成章²・ 長尾博文²・ 豊田岐聡²

A new tandem time-of-flight (TOF) mass spectrometer applicable to HE-ETD has been designed and is constructed. It consists of an electrospray ion source, an orthogonal acceleration, a MULTUM TOF spectrometer, a collision cell which can be filled with an alkali metal target, and a quadratic-field ion mirror. HE-ETD spectra provide the sequence information of all amino acid residues of doubly protonated vasopressin which has a disulfide linkages in the peptide, while HE-CAD and LE-ETD cannot provide the complete sequence information of the peptide.