1B-W1-1755 PDF
痒い所に手が届く質量分析計の使い方：概日時計研究を例として
State-of-the-art proteomics approach is becoming a general method chosen at the bench. Mass spectrometry has been a standard tool for the analysis for post-translational modification of proteins. MS-based quantification with a wide dynamic range is used to overcome a limitation of the “quantitative” western blotting assay. The next challenge is how to elucidate a physiological significance of dynamic protein turnover, modification, and post-translational modification based on a large proteomics dataset. We have designed a series of experiment by combining a MS-based identification of phosphorylation sites on a protein important for mammalian-circadian clock and functional rescue experiments in cultured cell and mice. Thus, a significance of identified post-translational modification is seamlessly analyzed in vivo.