演題概要

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第１日　５月１８日（水）　18:10～18:30　A会場（オービットホール）

1A-W1-1810　　PDF

LC/MS分析におけるマトリクス効果とLC分離

(¹島津・ ²日本ウォーターズ・ ³アジレント・ ⁴AB SCIEX・ ⁵ブルカーダルトニクス・ ⁶サーモフィッシャー)
^o塚本多矩¹・ 川畑慎一郎¹・ 佐藤太²・ 江崎達哉²・ 芹野武³・ 柴田猛⁴・ 瀧浪欣彦⁵・ 窪田雅之⁶

Given the growing LC/MS performance, ability and usability, LC/MS is becoming popular in various fields as a highly sensitive and selective analytical method to detect very small amount of target compounds in samples including complex matrices without complicated sample preparations. Though easy sample preparation relying on LC/MS selectivity increase laboratory efficiency, validation for sample matrix effect is necessary to assure accuracy and precision of analytical result.
Well known matrix effects are ion-enhancement and ion-suppression at MS ion source but matrices also affect separation at LC and cause peak shape distortion and retention time shift. To avoid these matrix effect, sample dilution and SPE is popular, however in some cases changing LC analytical condition or separation mode also decrease matrix effect dramatically.
In this workshop, we introduce about a example of matrix effect improvement for pesticide analysis in food sample.