演題概要

ポスター発表

第1日 5月14日(水)  会場(うめ)

内径100 マイクロメータ のピペットチップを用いた ナノエレクトロスプレーイオン化法

(山梨大学)
oチェンリーチュインRahman, Md. Matiur平岡賢三

Nano electrospray with solution flow rate below 100 nL/min is usually performed using metalized pulled glass capillary with tip inner diameter of < 10 μm. Here, we presents a new concept in realizing nano electrospray ionization mass spectrometry with a solution flow rate of ~10 nL/min using disposable plastic tips with inner diameter of ~100 μm. The inner and outer diameters were much larger than those of standard nanoESI emitters. However, it was possible to achieve a solution flow rate of ~10 nL/min for aqueous solution with these large emitters by conducting the electrospray under super-atmospheric pressure.
Besides the space charge effect, the high pressure condition also ensured the absence of electrical discharge, which was essential for a stable electrospray of aqueous solution. ESI with these low cost tips was equally sensitive as the standard nanoESI emitters and capable of producing long lasting signal from small amount of sample liquid. Due to a small initial droplet size under low flow rate regime, ESI with the present method also exhibited the property of nanoESI where the ion suppression of hydrophilic analyte by the hydrophobic compound presence in the solution was greatly reduced. Furthermore, the large inner diameter of the pipette tip also makes it easier to load the liquid sample and it is eased from the clogging problem faced by the standard nanoESI emitter.