1P-19 PDF
Searching the interaction of the analyte with Z- and E-sinapinic acid
(Ehime University1, University of Buenos Aires2)
Keywords: UV-MALDI MS, Z-Sinapinic acid, E-Sinapinic acid, Pressure Probe, Sample preparation
Because the presence of a rigid double bond in its molecule structure cinnamic acids can exist as two different geometric isomers, the E- and Z-forms. Commercial available cinnamic acids currently used as matrices are the trans- or E- isomer (E-cinnamic acids or trans-cinnamic acids). As a new rational design of MALDI matrices, Z-cinnamic acids were synthesized and their properties as matrices studied. Their performance was compared with that of the corresponding E-isomer and classical crystalline matrices. Thus, we demonstrated the outstanding performance for Z-SA. To better understand the quite different performance of each geometric isomer as matrix the physical and morphological properties as well as the photochemical stability and photophysics in solid state were studied. Fluorescence microscopy for solid surface scanning and MALDI-MS imaging of the same solid sample surface were complementary used. For sample preparation the picotapa method was used and the picoL volume was manipulated with the Pressure Probe.