1P-03 PDF
DBS法の有用性の評価 ―光に不安定な化合物の安定化―
The dried blood spots (DBS) has widely been used in drug development. DBS offers simpler sample collection and storage and requires a smaller blood volume. DBS is also used to stabilize unstable compounds in Bioanalysis.
In this study, we examined the usefulness of DBS in stabilizing light sensitive compounds. The stability under light (1500 lx, r.t., 24 h) of nifedipine, nisoldipine, manidipine, nitrendipine and lomefloxacin was determined in human plasma and blood, and on DBS cards and dried plasma spot (DPS) cards. Plasma and blood samples were deproteinized with acetonitrile. Spots on DBS and DPS cards were punched out and the compounds were extracted with organic solvents. The combination of cards (DMPK-A, B and C) and four solvents (containing methanol and/or acetonitrile) was optimized for each compound. UPLC (Waters)/TQ5500 (AB SCIEX) was used for analysis.
Percentage remaining of the parent compound of the five tested compounds in plasma, blood and DPS and DBS cards was 1 to 75%, 60 to 106%, 1 to 100% and 82 to 102%, respectively. Three of five light sensitive compounds were stable in blood and on DPS cards, and five compounds were stable on DBS cards.
This study demonstrated usefulness of the DBS method.