JMSSJ On-line Abstracts, Vol.47, No.4 (1999)

Recent Progress of Combinatorial Chemistry

Koichi FUKASE^* (^*Department of Chemistry, Guraduate School of Science, Osaka University, Machikaneyama 1-1, Toyonaka 560-0043, Japan)

J. Mass Spectrom. Soc. Jpn., 47(4), 251-269, 1999

This review describes the recent progress of combinatorial chemistry for drug discovery. Application of combinatorial chemistry is also described to functional studies of biologically active natural products including ourstudiesofbacterialglycoconjugatelipidA.LipidAanalogsweresynthesizedviaanewdivergentsynthetic route in order to clarify the biological importance of acyl groups. The number and chain length of the fatty acyl groups proved to have crucial influence on the biological activity. The present divergent route opens an efficient way toward the synthesis of lipid A libraries.

ESI-MS Analysis of Tetrapyridinium Macrocycle Complexationwith Carboxylic Anions

Tsutomu ABURA,^a) Tsuyoshi FUKUO,^a) Satoshi SHINODA,^b) Hiroshi TSUKUBE^b) and Ryuichi ARAKAWA^*a) (^*a)Department of Applied Chemistry, Kansai University, Suita, Osaka 564-8680, Japan, ^b)Department of Chemistry, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan)

J. Mass Spectrom. Soc. Jpn., 47(4), 270-273, 1999

Complex formations of a quaternary tetrapyridinium macrocycle (Cpy4Br₄) with anionic species, mono-, di-, tri-carboxylic acids, adenocine 5'-triphosphate (ATP), diphosphate (ADP), and monophosphate (AMP) were observed in a neutral pH region. Electrospray ionization mass spectrometry (ESI-MS) was used to study reactivity and selectivity of the complex formations between the Cpy4 cation and the guest anions.

Application of TLC-MALDI/TOFMS to Identification of Unknown Mixtures Produced in an Organic Synthetic Process

Kozo MATSUMOTO,^a) Shigeki HABAUE,^a) Hiroharu AJIRO,^a) and Yoshio OKAMOTOT^*a) (^*a)Department of Applied Chemistry, Guraduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan)

J. Mass Spectrom. Soc. Jpn., 47(4), 274-280, 1999

A TLC-MALDI/TOFMS system is developed for rapid identification of compounds in a mixture produced in an organic synthetic chemistry. Styrene oligomer is used as a test sample for obtaining the optimum experimental conditions. This system may be applied successfully to rapid identification of a new synthesized compound.

Analysis of Micelle Weight by ESI-MSA

Daisuke NOHARA^* (^*Department of Chemical Reaction Engineering, Faculty of Pharmaceutical Science, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan)

J. Mass Spectrom. Soc. Jpn., 47(4), 281-286, 1999

In mass spectrometry, electrospray ionization (ESI) method made it possible to measure the molecular weight of biopolymer accurately by producing multiply charged ions of the target molecule from the solution phase. Moreover, due to the softness of this method, the molecular weight of aggregates formed by noncovalent interactions can be measured. This article explains the applicability of ESI method to measurement of the micelle weight and the method of analysis of observed results.